Differential expression analysis of Liprin-a2 in hibernating bat (Rhinolophus ferrumequinum)

分类： 生物学 >> 生物医药 提交时间： 2017-05-10

Lihong Yuan Xueguo Zuo Jinping Chen Benfu Lin Junpeng Zhang Min SunShuyi Zhang

摘要： A PCR-based subtractive hybridization technique was used to identify genes up-regulated in the hibernating bat brain to explore the molecular mechanism of hibernation. Three genes, Liprin-a2, PTP4A2 and CAMKKb were differentially expressed in hibernating bat brain tissue compared to active bat brain tissue. One of them, Liprin-a2, which has recently been shown to have the key function in the organization of presynaptic and postsynaptic multiprotein complexes was studied in detail. We demonstrated that the expression level of Liprin-a2 was up-regulated almost 4-fold in hibernating bat brains by RT-PCR compared to levels in active bats. The differential expression pattern of Liprin-a2 was also detected in muscle, fat, brain and heart tissue of hibernating bats by real-time quantitative PCR. The result indicated that Liprin-a2 was over-expressed in brain and heart tissue and down-regulated in muscle and fat. In brain tissue of hibernating bats, Liprin-a2 expression was statistically significantly higher than in brain tissue of active controls (P = 0.029). The precise control of transcriptional level and the distinctively differential expression pattern of Liprin-a2 in different organs during circannual hibernation may have important physiological significance, not only in maintaining normal function of many key organs but also in effectively conserving limited energy resources without physiological damage.

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